Abstract: This paper puts forward an initial in-situ stress inversion method based on numerical simulation, taking into account the tunnel excavation disturbance along a plate′s suture zone, and has applied the method in five typical tunnels along a railway line. The results show that the correction coefficients k and λ of the in-situ stress for a typical tunnel along this railway vary between 1.236~1.404 and 0.846~1.069, respectively, and the actual measured values of the in-situ stress are generally small. Also, the results indicate that horizontal principal stresses have a substantial impact on tunnel construction, and it is necessary to carry out the inversion of initial in-situ stress for the construction of similar projects. Furthermore, according to this inversion method, if taking into account the excavation disturbance, the error between the calculated and measured values of the in-situ stress is no more than 5%.