Abstract: Tianshan Shengli Tunnel on Urumqi-Yuli Expressway in the Xinjiang Uygur Autonomous Region is 22.13 km long, which is the longest highway tunnel under construction in the world, and the project is facing a number of challenges such as construction of 20 km super long and deep highway tunnel under the influence of high cold, high altitude and other complex and harsh environments. As the first three-tube tunnel using TBM-assisted construction in the domestic highway industry, this paper systematically introduces innovative key construction technologies in terms of the construction organization, material transportation, construction ventilation, TBM construction, shaft operation, etc. and innovatively proposes new technologies, techniques and equipment including the threetube tunnel construction organization for "long tunnel construction by multi working faces", TBM trackless material transport, TBM pressurized pumping concrete process, suspended continuous belt conveyor on the roof, multi-working face construction ventilation, deep pre-grouting on the ground surface of the deep shaft, short excavation with short lining operation and others, which will provide a systematic reference for the future construction of ultra-long tunnels,and is of great significance for upgrading the construction level of the ultra-long tunnels.