Abstract: The relationship between relative maximum oxygen uptake and personnel evacuation exercise ability is obtained according to the completion time of different subjects in the 500 m evacuation test and in combination with the relative maximum oxygen uptake in the step test. The subjects participating in the test are between 20 and 25 years old and a total of 13 subjects are selected for the step test and evacuation test, respectively. In the step test,physiological parameters, e.g. blood oxygen saturation and heart rate of the subjects at different stages, are tested and the relative comprehensive heart rate is proposed to evaluate the exercise ability. The maximum oxygen uptake of the subjects is indirectly converted by the A-R nomogram method, and the relative maximum oxygen uptake is used to eliminate individual differences. 48 hours after the step test, the same subjects are asked to conduct a 500 m evacuation test, of which the completion time is recorded accordingly. Then, the fitting analysis is carried out for relative maximum oxygen uptake obtained from the step test and completion time of the evacuation test. It is found that there is an inverse relationship between the completion time of the evacuation test and the relative maximum oxygen uptake. So, the relative maximum oxygen uptake can be used to indirectly express the evacuation exercise ability.