Abstract: The beam-spring model is widely used in the calculation of segment lining structure. As for the joint stiff? ness there are mainly the model test method and the empirical method, of which the former is time-consuming and costly, while the latter is inevitably subjective to a certain extent. In view of this, this paper develops an inversion analysis program for the displacement of shield tunnel segment structure based on genetic algorithm by using C++ programming language. By inverse calculation examples of elastic modulus, the error between the inversion value and the theoretical value is 1.4%, verifying the feasibility of the algorithm and program. Using the program, the inversed joint stiffness values are compared with the segment joint stiffness values in actual design cases, the inversion calculation results show that the inversion error of the rotational stiffness of the circumferential segment joint is 2.6% under positive bending moment, and 4.3% under the condition of negative bending moment.